NDLI: Novel nanostructured platform and nanoparticles for sensitive detection of biological materials

Content Provider	IEEE Xplore Digital Library
Author	Sangho Bok Dasgupta, P.K. Korampally, V. Polo-Parada, L. Folk, W. Gangopadhyay, K. Gangopadhyay, S.
Copyright Year	2010
Description	Author affiliation: Department of Electrical Engineering, University of Missouri, Columbia, 65211, USA (Korampally, V.; Gangopadhyay, K.; Gangopadhyay, S.) \|\| Nanos Technologies LLC., Columbia, MO 65203, USA (Sangho Bok) \|\| Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, 65211, USA (Polo-Parada, L.) \|\| Department of Chemistry and Biochemistry, University of Texas at Arlington, 76019, USA (Dasgupta, P.K.) \|\| Department of Biochemistry, University of Missouri, Columbia, 65211, USA (Folk, W.)
Abstract	In this paper, a simple and robust sensing assay platform for detection of biological materials is reported. The fluorescence based sensing platform benefits from the combined use of our novel dye-doped nanoparticle tags and nanoporous high surface area (∼1,400 $m^{2}/g)$ films to achieve high intrinsic signal amplification. The dye-doped nanoparticles are synthesized from poly-methylsilsesquioxane (PMSSQ) encapsulating fluorescent dye molecules that can be conjugated to antibodies and proteins. We have achieved conjugation of up to 64 dye molecules per single antibody, which represents more than an order of magnitude increase in conjugation efficiency compared to conjugation of free dye molecules. Capture of the dye-doped nanoparticle labeled antibodies by nanoporous organosilicate (NPO) films has yielded 540 fold increase in fluorescence response compared to immobilization of dye labeled antibodies on (flat) glass substrates. This assay demonstrates a general way for detection of analytes in very low concentration.
Starting Page	450
Ending Page	453
File Size	459680
Page Count	4
File Format	PDF
ISBN	9781424481705
ISSN	19300395
e-ISBN	9781424481699
e-ISBN	9781424481682
DOI	10.1109/ICSENS.2010.5690180
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-11-01
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Nanoparticles Films Proteins Substrates Sensors Glass
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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