NDLI: Microfabricated device for co-culture of sympathetic neuron and iPS-derived cardiomyocytes

Content Provider	IEEE Xplore Digital Library
Author	Takeuchi, A. Shimba, K. Takayama, Y. Kotani, K. Jong-Kook Lee Noshiro, M. Jimbo, Y.
Copyright Year	2013
Description	Author affiliation: Dept. of Cardiovascular Regenerative Med., Osaka Univ., Suita, Japan (Jong-Kook Lee) \|\| Grad. Sch. of Frontier Sci., Univ. of Tokyo, Kashiwa, Japan (Takeuchi, A.; Shimba, K.; Kotani, K.; Jimbo, Y.) \|\| Kitasato Univ., Sagamihara, Japan (Noshiro, M.) \|\| Grad. Sch. of Inf. Sci. & Technol., Univ. of Tokyo, Tokyo, Japan (Takayama, Y.)
Abstract	Induced pluripotent stem (iPS) cell-derived cardiomyocytes (iPS-CMs) has been expected as a cell source for therapy of serious heart failure. However, it is unclear whether the function of iPS-CMs is modulated by the host sympathetic nervous system. Here we developed a device for co-culture of sympathetic neurons and iPS-CMs using microfabrication technique. The device consisted of a culture chamber and a microelectrode-array (MEA) substrate. The superior cervical ganglion (SCG) neurons were co-cultured with iPS-CMs in a microfabricated device, which had multiple compartments. Several days after seeding, synapses were formed between SCG neurons and iPS-CMs, as confirmed by immunostaining. Spontaneous electrical activities of the SCG neurons and the iPS-CMs were observed from the electrode of the MEA substrate. The beat rate of iPS-CMs increased after electrical stimulation of the co-cultured SCG neurons. Such changes in the beat rate were prevented in the presence of propranolol, a β-adrenoreceptor antagonist. These results suggest that the microfabricated device will be utilized for studying the functional modulation of iPS-CMs by connected sympathetic neurons.
Starting Page	3817
Ending Page	3820
File Size	872504
Page Count	4
File Format	PDF
ISBN	9781457702167
ISSN	1557170X
DOI	10.1109/EMBC.2013.6610376
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-07-03
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Neurons Electrodes Substrates Electrical stimulation Reservoirs Aggregates Microchannel
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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