NDLI: Precise 3-D localization of fluorescent probes without numerical fitting

Content Provider	IEEE Xplore Digital Library
Author	Ting Sun Andersson, S.B.
Copyright Year	2007
Description	Author affiliation: Boston Univ., Boston (Ting Sun; Andersson, S.B.)
Abstract	We present an analytical algorithm for determining the position of a fluorescent probe in three dimensions (3-D) from a collection of measurements taken at different locations. This algorithm, fluoroBancroft, relies on the fact that the intensity point spread function depends only on the radial distance between the center of the focal point and the position of the fluorescent probe. We present a simulation study comparing the performance of the algorithm to the standard technique of fitting the data to a Gaussian profile. Our results indicate that in the 3-D case, the fluoroBancroft algorithm is able to localize the probe with an accuracy on the order of tens of nanometers using less than ten measurements (pixels). The Gaussian fitting procedure is unable to locate the probe even when using 36 measurements (the maximum number of measurements used in the simulations). Moreover the new technique is typically two orders of magnitude faster than the Gaussian fitting approach in terms of computation time. These results indicate the fluoroBancroft algorithm can be used effectively in a closed-loop controller to track the motion of single fluorescent probes in 3-D in a confocal microscope.
Starting Page	4181
Ending Page	4184
File Size	774233
Page Count	4
File Format	PDF
ISBN	9781424407873
ISSN	1557170X
DOI	10.1109/IEMBS.2007.4353258
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-08-22
Publisher Place	France
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Probes Microscopy Position measurement Sun Particle tracking Algorithm design and analysis Computational modeling Motion control Image analysis
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

PRECISE LOCALIZATION OF FLUORESCENT PROBES WITHOUT NUMERICAL FITTING

Precise localization of fluorescent probes without numerical fitting.

Position estimation of fluorescent probes in a confocal microscope

Signal source localization with tetrodes: Experimental verification

Evaluation of autofocus algorithms for tuberculosis microscopy

Motion detection for subcellular structure trafficking

A nanoelectromechanical biosensor based on precise quantification and control of DNA orientation

Subcellular particles tracking in time-lapse confocal microscopy images

Cell motion analysis without explicit tracking

Precise 3-D localization of fluorescent probes without numerical fitting

Similar Documents

PRECISE LOCALIZATION OF FLUORESCENT PROBES WITHOUT NUMERICAL FITTING

Precise localization of fluorescent probes without numerical fitting.

Position estimation of fluorescent probes in a confocal microscope

Signal source localization with tetrodes: Experimental verification

Evaluation of autofocus algorithms for tuberculosis microscopy

Motion detection for subcellular structure trafficking

A nanoelectromechanical biosensor based on precise quantification and control of DNA orientation

Subcellular particles tracking in time-lapse confocal microscopy images

Cell motion analysis without explicit tracking

Precise 3-D localization of fluorescent probes without numerical fitting