NDLI: Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals

Content Provider	IEEE Xplore Digital Library
Author	Osman, A. Joon Hyuk Park Dickensheets, D. Platisa, J. Culurciello, E. Pieribone, V.A.
Copyright Year	2007
Abstract	In this paper we present a fully self-contained imaging instrument (30 mm overall length) that is capable of recording high speed and detect relatively small fluorescent signals (0.1% ΔF/F) from brain tissues potentially containing genetically-encoded sensors or dyes. This device potentially enables the study of neuronal activity in awake and mobile animals during natural behaviors without the stress and suppression of anesthesia and restraint. The device is a fully self-contained illumination system, wide field fluorescence microscope ( ~ 4.8mm² FOV-25 um lateral resolution-1.8 × magnification-0.39 NA) and CMOS image sensor (32 × 32). The total weight of the system is 10 g and is capable of imaging up to 900 fps. We present voltage dye RH1692 experiments using the system to study the somatosensory cortex of mice during whisker movements using an air puff.
Sponsorship	IEEE Circuits and Systems Society IEEE Engineering in Medicine and Biology Society
Page Count	8
File Size	1509254
Starting Page	446
Ending Page	453
File Format	PDF
ISSN	19324545
Volume Number	6
Issue Number	5
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microscopy Optical imaging Optical sensors Lenses Light emitting diodes fluorescent microscope Biomedical optical imaging brain imaging CMOS imaging sensor
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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