NDLI: Comparison of the behavior of fibroblast and bone marrow-derived mesenchymal stem cell on nitrogen plasma-treated gelatin films.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Prasertsung, I. Kanokpanont, S. Mongkolnavin, R. Wong, C. S. Panpranot, J. Damrongsakkul, S.
Description	Country affiliation: Thailand Author Affiliation: Prasertsung I ( Chemical Engineering Program, Department of Industrial Engineering, Faculty of Engineering, Naresuan University, Phitsanulok 65000, Thailand.)
Abstract	The attachment and growth behavior of mouse fibroblast (L929) and rat bone marrow-derived mesenchymal stem cell (MSC) on nitrogen plasma-treated and untreated gelatin films was investigated and compared. The gelatin films were prepared by solution casting (0.05% w/v) and crosslinked using dehydrothermal treatment. The crosslinked gelatin films were treated with nitrogen alternating current (AC) 50 Hz plasma systems at various treatment time. The results on the attachment and growth of two cells; L929 and MSC, on plasma-treated gelatin film showed that the number of attached and proliferated cells on plasma-treated gelatin films was significantly increased compared to untreated samples. However, no significant difference between the number of attached L929 and MSC on plasma-treated gelatin was observed. The shorter population doubling time and higher growth rate of cells cultured on plasma-treated film indicated the greater growth of cells, compared to ones on untreated films. The greatest enhancement of cell attachment and growth were noticed when the film was treated with nitrogen plasma for 9 to 15s. This suggested that the greater attachment and growth of both cells on gelatin films resulted from the change of surface properties, i.e. hydrophilicity, surface energy, and chemistry. The suitable water contact angle and oxygen/nitrogen ratio (O/N) of gelatin film for best L929 and MSC attachment were observed at 27-32° and 1.4, respectively. These conditions also provided the best proliferation of cells on plasma-treated gelatin films.
File Format	HTM / HTML
ISSN	09284931
Issue Number	7
Volume Number	33
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2013-10-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Bone Marrow Cells Cytology Fibroblasts Gelatin Pharmacology Mesenchymal Stromal Cells Nitrogen Plasma Gases Animals Drug Effects Metabolism Ultrastructure Cell Adhesion Cell Proliferation Cell Shape Cells, Cultured Female Mice Rats Rats, Wistar Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject Domain (in MeSH)	Cells Eukaryota Inorganic Chemicals Amino Acids, Peptides, and Proteins Cell Physiological Phenomena
Subject	Biomaterials Mechanical Engineering Condensed Matter Physics Mechanics of Materials Bioengineering

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