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Content Provider | SpringerLink |
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Author | Ayyildiz Tamis, Duygu Avcı, Kamuran Deliloglu Gurhan, S. Ismet |
Copyright Year | 2013 |
Abstract | Microcarriers provide large adhesion area allowing high cell densities in bioreactor systems. This study focused on the investigation of cell adhesion and cell growth characteristics of both anchorage-dependent CHO-K1 and anchorage-independent Ag8 myeloma cell lines cultivated on four different microcarriers (Biosilon®, Microhex®, Cytodex 3®, Cytoline 2®) by considering the cell kinetics and physiological data. Experiments were performed in both static and agitated cell culture systems by using 24-well tissue culture plates and then 50-ml spinner flasks. In agitated cultures, the highest specific growth rates (0.026 h for CHO-K1 and 0.061 h for Ag8 cell line) were obtained with Cytodex 3® and Cytoline 2® microcarriers for CHO-K1 and Ag8 cell line, respectively. Metabolic characteristics showed some variation among the cultures with the four microcarriers. The most significant being the higher production of lactate with microcarriers with CHO-K1 cells relative to the Ag8 cells. SEM analyses revealed the differences in the morphology of the cells along with microcarriers. On Cytodex 3® and Cytoline 2®, CHO-K1 cells attached to the substratum through long, slender filopodia, whereas the cells showed a flat morphology by covering the substratum on the Biosilon® and Microhex®. Ag8 cells maintained their spherical shapes throughout the culture for all types of microcarriers. In an attempt to scale-up, productions were carried out in 50-ml spinner flasks. Cytodex 3® (for CHO-K1 cells) and Cytoline 2® (for Ag8 cells) were evaluated. The results demonstrate that high yield of biomass could be achieved through the immobilization of the cells in each culture system. And cell cultures on microcarriers, especially on Cytodex 3® and Cytoline 2®, represented a good potential as microcarriers for larger scale cultures of CHO-K1 and Ag8, respectively. Moreover, owing to the fact that the cell lines and culture media are specific, outcomes will be applicable for other clones derived from the same host cell lines. |
Starting Page | 221 |
Ending Page | 231 |
Page Count | 11 |
File Format | |
ISSN | 10712690 |
Journal | In Vitro Cellular & Developmental Biology - Animal |
Volume Number | 50 |
Issue Number | 3 |
e-ISSN | 1543706X |
Language | English |
Publisher | Springer US |
Publisher Date | 2013-12-20 |
Publisher Place | Boston |
Access Restriction | One Nation One Subscription (ONOS) |
Subject Keyword | CHO-K1 Ag8 myeloma Microcarrier Immobilization Spinner flask Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics |
Content Type | Text |
Resource Type | Article |
Subject | Cell Biology Developmental Biology Medicine |
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