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Temporal Focusing with Structured Illumination Can Be Applied for High-frame-rate, Wide-field Optical-sectioning Multiphoton Microscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Holland, Daniel B. Blake, Geoffrey A. Guo, Chin-Lin |
| Copyright Year | 2013 |
| Abstract | Temporal focusing, an effect in which the effective pulse duration of an ultrafast pulse train is a function of propagation distance [1], was expected to be a solution for high-frame-rate optical-sectioning microscopy upon its invention. However, two issues of conventional temporal-focusing microscopy confined its applications. First, it is difficult for multiwavelength applications because the optical path of the system depends on light wavelengths, owing to the use of diffraction gratings to create the time delays required for temporal focusing. Second, its axial resolution is moderate because the amount of time delay created by a single grating is insufficient. Multiple-grating optical designs have been proposed to enhance axial resolution [2], but such a method makes multi-wavelength applications even more difficult. Here we propose a novel design using a special microlens array to generate temporal focusing, along with a high-spatial-frequency illumination pattern (Fig. 1(b), HSMA). In this microlens array, individual microlenses are of various heights (referred to as a height-staggered microlens array, HSMA) and can thus generate time delays with respect to one another. Unfortunately, our estimation suggests that the time delay required for optimal axial resolution, through height-staggering alone, is too large for any practical designs of HSMA. In order to enhance the axial resolution with practical optical designs, we propose to utilize the illumination pattern by introducing structured illumination process [3] (Fig. 1(b), HSMA + structured illumination). The results of numerical simulations show that our proposed method can provide finer axial resolution than conventional confocal microscopy. We believe such a simple design with exceptional performance can become a powerful tool for biomedical imaging in the future. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://focusonmicroscopy.org/2013/PDF/169_Yu.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
