NDLI: Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

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Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

Content Provider	Scilit
Author	Raffoul, Tássia Swiech, Kamilla Arantes, Mabel Karina de Sousa, Álvaro Paiva Braga Mendonça, Ronaldo Zucatelli Pereira, Carlos Augusto Suazo, Cláudio Alberto Torres
Copyright Year	2005
Description	The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.
Related Links	http://www.scielo.br/pdf/babt/v48nspe/25282.pdf
Ending Page	95
Page Count	11
Starting Page	85
ISSN	15168913
e-ISSN	16784324
DOI	10.1590/s1516-89132005000400011
Journal	Brazilian Archives of Biology and Technology
Issue Number	spe
Volume Number	48
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2005-06-01
Access Restriction	Open
Subject Keyword	Brazilian Archives of Biology and Technology Agriculture and Animal Production
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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