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Content Provider | IEEE Xplore Digital Library |
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Author | Pastorino, L. Soumetz, F.C. Ruggiero, C. |
Copyright Year | 2007 |
Description | Author affiliation: Dept. of Commun., Comput. & Syst. Sci., Genova Univ. (Pastorino, L.; Soumetz, F.C.; Ruggiero, C.) |
Abstract | The immobilization of two different immunoglobulins by means of the layer by layer self assembly technique (LbL) was studied and evaluated for its use in the development of piezoelectric biosensors. Namely an antibody specific to transforming growth factor pi (anti-TGF-pl) and one specific to taxol were immobilized onto the surface of piezoelectric crystals by the LbL technique and their performance was characterized. The quartz crystal microbalance technique was employed to characterize both the step by step immobilization procedure and the response to the specific analytes. Atomic force microscopy was used to measure the roughness of the crystals before and after immunoglobulin immobilization. The results showed that the proposed immunosensors offer a promising alternative to classical analytical methods for a fast and easy determination of analytes of clinical interest. |
Starting Page | 29 |
Ending Page | 32 |
File Size | 4833194 |
Page Count | 4 |
File Format | |
ISBN | 1424406102 |
DOI | 10.1109/NEMS.2007.352033 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2007-01-16 |
Publisher Place | Thailand |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Self-assembly Immune system Biosensors Crystals Atomic force microscopy Nonhomogeneous media Atomic measurements Biochemical analysis Chemical elements Chemical transducers atomic force microscopy Layer by Layer self assembly quartz crystal microbalance immunosensor antibody |
Content Type | Text |
Resource Type | Article |
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