NDLI: Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition

Content Provider	IEEE Xplore Digital Library
Author	Beebe, S.J. Fox, P.M. Rec, L.J. Somers, K. Stark, R.H. Schoenbach, K.H.
Copyright Year	2001
Description	Author affiliation: Dept. of Pediatrics, Eastern Virginia Med. Sch., Norfolk, VA, USA (Beebe, S.J.; Fox, P.M.; Rec, L.J.; Somers, K.)
Abstract	Pulse power technology using high intensity (up to 300 kV/cm) nanosecond pulsed electric fields (nsPEF) has been applied for decontamination and amelioration of biofouling, but until now effects on human cells have not been investigated. To analyze structural and functional changes in human cells and solid tumors following exposure to nsPEF we utilized flow cytometry and immunofluorescence microscopy. We provide further support for the hypothesis that as the pulse duration is decreased, there is a lower incidence of electric field interactions at the plasma membrane and a higher incidence of interactions with intracellular structures. The nsPEF effects are pulse duration/electric field intensity-dependent and energy density- or temperature-independent. We also show that nsPEF induces programmed cell death (apoptosis) in cultured cells as indicated by cell shrinkage, Annexin-V-FITC binding to phosphatidylserine on intact cells, and caspase activation. Mouse fibrosarcoma tumors exposed to nsPEF exhibit fragmented DNA and reduced tumor growth in a mouse model. These studies show that nsPEF effects are distinctly different than electroporation pulses and provide the first evidence for the potential application of nsPEF to induce apoptosis and inhibit tumor growth.
Sponsorship	Plasma Sci. & Appl. Committee Pulsed Power Sci. & Technol. Committee of the IEEE Nucl. & Plasma Sci. Soc
Starting Page	211
Ending Page	215
File Size	594582
Page Count	5
File Format	PDF
ISBN	0780371208
DOI	10.1109/PPPS.2001.1002030
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2001-06-17
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Neoplasms Cells (biology) Humans Mice Decontamination Solids Immune system Microscopy Plasma density Plasma temperature
Content Type	Text
Resource Type	Article

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