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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Tak Kit Lau Yun-hui Liu Yu-jie Lu Chi-ming Cheuk Kai-wun Lin |
| Copyright Year | 2013 |
| Description | Author affiliation: Dept. of Mech. & Autom. Eng., Chinese Univ. of Hong Kong, Hong Kong, China (Tak Kit Lau; Yun-hui Liu; Yu-jie Lu; Chi-ming Cheuk; Kai-wun Lin) |
| Abstract | In this paper, we present a novel design of low-cost, high-throughput hologram-based 3D imaging system towards the development of a detection system for Escherichia coli (E. coli). This focusing-free system can image food samples flowing in a transparent microchannel under illumination of RGB lasers through a pinhole, and yields their 3D structures in real-time using hologram-based reconstruction algorithms. We solve two key technical problems in order to realize the system: 1) implementation of a high-speed (200fps) imaging system for capturing full-frame holograms of cells flowing in microchannels; 2) reconstruction of 3D structure of E. coli by characterizing the holograms under low-cost incoherent light sources such as the incoherent laser diodes. Market-dominating food pathogen detection systems using the polymerase chain reaction (PCR) are very slow (10-20 hours/ml) in addition to their high costs. Moreover, they cannot detect the viable but non-culturable (VBNC) E. coli, which orchestrated the recent massive and deadly food poisoning outbreaks in Europe and North America. The estimated cost of the proposed system is less than US$11,000 and its speed is over 1,000 times faster than the existing methods. |
| Sponsorship | IEEE Nanotechnol. Counc. |
| Starting Page | 726 |
| Ending Page | 731 |
| File Size | 1347773 |
| Page Count | 6 |
| File Format | |
| ISSN | 19449399 |
| e-ISBN | 9781479906765 |
| DOI | 10.1109/NANO.2013.6720887 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2013-08-05 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Image reconstruction Three-dimensional displays Holography Real-time systems Reconstruction algorithms Lenses |
| Content Type | Text |
| Resource Type | Article |
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