NDLI: Nanophotonics for live cell observation with high resolution

Content Provider	IEEE Xplore Digital Library
Author	Inami, W. Nawa, Y. Kawata, Y.
Copyright Year	2013
Description	Author affiliation: Dept. of Mech. Eng., Shizuoka Univ., Hamamatsu, Japan (Nawa, Y.; Kawata, Y.) \|\| Div. of Global Res. Leaders, Shizuoka Univ., Hamamatsu, Japan (Inami, W.)
Abstract	We present superresolving optical imaging system and demonstrate the observation of biological specimens without any stained process. The electron-beam excited assisted (EXA) optical microscope has a few tens nanometer spatial resolution laterally and is possible to observe dynamic behaviors of specimens in various surroundings such as air or liquids. In the EXA-microscope, a nano-light source in a few nanometers size is excited by focused electron beam in an emission layer. An electron beam can be focused to a spot size as small as 1 nanometer in diameter. The EXA-microscope enables to observe optical constants such as absorption, refractive index, polarization properties, and its dynamic behaviors in nanometer scale. We also have developed a direct electron-beam excitation assisted optical microscope with a resolution of a few tens of nanometers and it can be applied for observation of dynamic movements of nanoparticles in liquid. In the microscope, fluorescent materials are directly excited with a focused electron beam. In this paper we present the evaluation result of resolution and observation results of living HeLa cells with high resolution. We have successfully observed fine structures of the cells without any stain process. This is first demonstration of observation of intercellular granules in HeLa cells.
Starting Page	1
Ending Page	4
File Size	297066
Page Count	4
File Format	PDF
e-ISBN	9781467357852
DOI	10.1109/QiR.2013.6632524
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-06-25
Publisher Place	Indonesia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Scanning electron microscopy Optical microscopy Nano-imaging Electron beams Films Optical imaging Nanobioscience Cathodoluminescenc Near-field optical microscopy Electron scanning microscopy Bio-imaging
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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