NDLI: Monte Carlo Simulation of 3D Mapping of Cardiac Electrical Activity with Spinning Slit Confocal Optics

Content Provider	IEEE Xplore Digital Library
Author	Hwang, S.-M. Choi, B.-R. Salama, G.
Copyright Year	2006
Description	Author affiliation: Dept. of Cell Biol. & Physiol., Pittsburgh Univ., PA (Hwang, S.-M.)
Abstract	Optical techniques used to map transmembrane potential and intracellular $Ca^{2+}$ activities of intact hearts are restricted to the surface and cannot resolve activity in deeper layers due to the lack of depth resolution. The recent development of spinning slit confocal optics offers advantages of depth resolution as well as high-speed confocal imaging which are necessary for millisecond-scale, depth-resolved mapping of membrane potential and/or intracellular $Ca^{2+}$ concentration. Here, we show simulated confocal optics derived from confocal slits on a high-speed spinning disk using Monte Carlo method with a numerical heart tissue model and find that depth-resolved optical mapping is feasible down to around 800 mum below the surface using 670-nm excitation light. The numerical model shows that (1) a minimum slit separation, which is found to be a function of depth of the focal plane and the numerical aperture of the objective lens, for minimum background noise exists and (2) narrower slit widths result in slightly greater depth resolution but has a negative impact in significantly lower overall fluorescence intensity. An experimental test of this optics has been performed by imaging two overlapping layers of fluorescent beads and the result confirms the expected depth resolution in non-scattering medium. These results will be able to serve as a benchmark on how a 3D-imaging system can be expected to perform and what kind of theoretical depth-resolution can be expected from it
Sponsorship	IEEE EMB
Starting Page	1093
Ending Page	1097
File Size	704087
Page Count	5
File Format	PDF
ISBN	1424400325
ISSN	1557170X
DOI	10.1109/IEMBS.2006.259837
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Spinning High speed optical techniques Heart Image resolution Optical imaging Numerical models Optical noise Fluorescence High-resolution imaging Biomembranes
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Complete angle small animal fluorescence imaging with early-arriving photons

GPU acceleration of optical mapping algorithm for cardiac electrophysiology

High-speed spinning-disk interferometry on the BioCD for human diagnostic applications

Preprocessing of fluoresced transmembrane potential signals for cardiac optical mapping

MEMS-based 3D optical microendoscopy

Widefield fluorescence sectioning with HiLo microscopy

Automated Extended Volume Imaging of Tissue using Confocal and Optical Microscopy

Epicardial rotors in panoramic optical maps of fibrillating swine ventricles

Characteristics of motion artifacts in cardiac optical mapping studies

Monte Carlo Simulation of 3D Mapping of Cardiac Electrical Activity with Spinning Slit Confocal Optics

Similar Documents

Complete angle small animal fluorescence imaging with early-arriving photons

GPU acceleration of optical mapping algorithm for cardiac electrophysiology

High-speed spinning-disk interferometry on the BioCD for human diagnostic applications

Preprocessing of fluoresced transmembrane potential signals for cardiac optical mapping

MEMS-based 3D optical microendoscopy

Widefield fluorescence sectioning with HiLo microscopy

Automated Extended Volume Imaging of Tissue using Confocal and Optical Microscopy

Epicardial rotors in panoramic optical maps of fibrillating swine ventricles

Characteristics of motion artifacts in cardiac optical mapping studies

Monte Carlo Simulation of 3D Mapping of Cardiac Electrical Activity with Spinning Slit Confocal Optics