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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Luo, Huiying Yang, Peilong Yao, Bin Huang, Huoqing Meng, Kun Bai, Yingguo Wang, Guozeng Wang, Yaru Shi, Pengjun |
Description | Author Affiliation: Wang G ( Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.) |
Abstract | A xylanase-coding gene, xynGR40, was cloned directly from the environmental DNA of goat rumen contents and expressed in Escherichia coli BL21 (DE3). The 1446-bp full-length gene encodes a 481-residue polypeptide (XynGR40) containing a catalytic domain belonging to glycosyl hydrolase (GH) family 10. Phylogenetic analysis indicated that XynGR40 was closely related with microbial xylanases of gastrointestinal source. Purified recombinant XynGR40 exhibited high activity at low temperatures, and remained active (â ¼10% of the activity) even at 0°C. The optimal temperature of XynGR40 was 30°C, much lower than other xylanases from rumen. Compared with mesophilic and thermophilic counterparts, XynGR40 had fewer hydrogen bonds and salt bridges, and lengthened loops in the catalytic domain. The enzyme also had relatively better stability at mesophilic temperatures and a higher catalytic efficiency than other known GH 10 cold active xylanases. These properties suggest that XynGR40 is a novel cold active xylanase and has great potential for basic research and industrial applications. |
ISSN | 09608524 |
Issue Number | 3 |
Volume Number | 102 |
e-ISSN | 18732976 |
Journal | Bioresource Technology |
Language | English |
Publisher | Elsevier |
Publisher Date | 2011-02-01 |
Publisher Place | Great Britain (UK) |
Access Restriction | Subscribed |
Subject Keyword | Cloning, Molecular Methods Dna, Bacterial Genetics Isolation & Purification Endo-1,4-beta Xylanases Physiology Escherichia Coli Chemistry Metabolism Rumen Microbiology Amino Acid Sequence Animals Enzyme Activation Enzyme Stability Goats Male Molecular Sequence Data Temperature Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Animal Structures Eukaryota Bacteria Enzymes and Coenzymes Nucleic Acids, Nucleotides, and Nucleosides Investigative Techniques Physical Phenomena Chemical Phenomena Information Science |
Subject | Bioengineering Renewable Energy, Sustainability and the Environment Waste Management and Disposal Environmental Engineering Medicine |
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