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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Gao, Yuan Liu, Zi-Lu Song, Hui-Ting Yang, Yi-Min Xiao, Wen-Jing Xia, Wu-Cheng Jiang, Zheng-Bing Liu, Shi-Hui Yi, Li |
Description | Author Affiliation: Song HT ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China); Gao Y ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: gaoyuan0808@sina.cn.); Yang YM ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: wahy-vv@163.com.); Xiao WJ ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: vickichiu1992@sina.com.); Liu SH ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: liushihui1210@sina.com.); Xia WC ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: 13035135871@163.com.); Liu ZL ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: 1_zilulu@sina.com.); Yi L ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China. Electronic address: liyi@hubu.edu.cn.); Jiang ZB ( Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei University, Wuhan 430062, PR China) |
Abstract | Synergistic combination of cellulase and xylanase has been performed on pre-treated substrates in many previous studies, while few on natural substrates. In this study, three unpretreated lignocellulosic substrates were studied, including corncob, corn stover, and rice straw. The results indicated that when the mixed cellulase and xylanase were applied, reducing sugar concentrations were calculated as 19.53, 15.56, and 17.35mg/ml, respectively, based on the 3,5 dinitrosalicylic acid (DNS) method. Compared to the treatment with only cellulose, the hydrolysis yields caused by mixed cellulase and xylanase were improved by 133%, 164%, and 545%, respectively. In addition, the conversion yield of corncob, corn stover, and rice straw by cellulase-xylanase co-treatment reached 43.9%, 48.5%, and 40.2%, respectively, based on HPLC analysis, which confirmed the synergistic effect of cellulase-xylanase that was much higher than either of the single enzyme treatment. The substrate morphology was also evaluated to explore the synergistic mechanism of cellulase-xylanase. |
ISSN | 09608524 |
Journal | Bioresource Technology |
Volume Number | 219 |
e-ISSN | 18732976 |
Language | English |
Publisher | Elsevier |
Publisher Date | 2016-11-01 |
Publisher Place | Great Britain (UK) |
Access Restriction | Subscribed |
Subject Keyword | Discipline Bioresource |
Content Type | Text |
Resource Type | Article |
Subject | Bioengineering Renewable Energy, Sustainability and the Environment Waste Management and Disposal Environmental Engineering Medicine |
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