NDLI: Electrochemical deoxyribonucleic acid biosensor based on electrodeposited graphene and nickel oxide nanoparticle modified electrode for the detection of salmonella enteritidis gene sequence.

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Electrochemical deoxyribonucleic acid biosensor based on electrodeposited graphene and nickel oxide nanoparticle modified electrode for the detection of salmonella enteritidis gene sequence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sun, Wei Wang, Xiuli Lu, Yongxi Gong, Shixing Qi, Xiaowei Lei, Bingxin Sun, Zhenfan Li, Guangjiu
Description	Author Affiliation: Sun W ( College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China. Electronic address: swyy26@hotmail.com.); Wang X ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.); Lu Y ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.); Gong S ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.); Qi X ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.); Lei B ( College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.); Sun Z ( College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, PR China.); Li G ( College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China. Electronic address: lgjqust@126.com.)
Abstract	In this paper a new electrochemical DNA biosensor was prepared by using graphene (GR) and nickel oxide (NiO) nanocomposite modified carbon ionic liquid electrode (CILE) as the substrate electrode. GR and NiO nanoparticles were electrodeposited on the CILE surface step-by-step to get the nanocomposite. Due to the strong affinity of NiO with phosphate groups of ssDNA, oligonucleotide probe with a terminal 5'-phosphate group could be attached on the surface of NiO/GR/CILE, which could further hybridize with the target ssDNA sequence. Methylene blue (MB) was used as the electrochemical indicator for monitoring the hybridization reaction. Under the optimal conditions the reduction peak current of MB was proportional to the concentration of salmonella enteritidis gene sequence in the range from 1.0×10(-13) to 1.0×10(-6)molL(-1) with a detection limit as 3.12×10(-14)molL(-1). This electrochemical DNA sensor exhibited good discrimination ability to one-base and three-base mismatched ssDNA sequences, and the polymerase chain reaction amplification product of salmonella enteritidis gene sequences were further detected with satisfactory results.
File Format	HTM / HTML
ISSN	09284931
Volume Number	49
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2015-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Biosensing Techniques Dna Analysis Electrochemical Techniques Methods Graphite Chemistry Metal Nanoparticles Nickel Salmonella Enteritidis Genetics Genes, Bacterial Microscopy, Electron, Scanning Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject Domain (in MeSH)	Bacteria Inorganic Chemicals Nucleic Acids, Nucleotides, and Nucleosides Diagnosis Investigative Techniques Genetic Phenomena Technology, Industry, and Agriculture
Subject	Biomaterials Mechanical Engineering Condensed Matter Physics Mechanics of Materials Bioengineering

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