NDLI: Semi-automatic morphological measurements of 2-D and 3-D microvascular images

Content Provider	IEEE Xplore Digital Library
Author	Merchant, F.A. Aggarwal, S.J. Diller, K.R. Bovik, A.C.
Copyright Year	1994
Description	Author affiliation: Biomed. Eng. Program, Texas Univ., Austin, TX, USA (Merchant, F.A.; Aggarwal, S.J.; Diller, K.R.)
Abstract	The technique of serial optical sectioning by laser scanning confocal microscopy (LSCM), in conjunction with off-line digital image analysis, was used to quantize the morphological changes occurring during angiogenesis and revascularization of pancreatic islets transplanted at the renal subcapsular site in rats. The process consisted of in-vivo imaging of the microvasculature which was optically enhanced by the administration of a fluorescent probe into the circulating blood. Serial two-dimensional (2-D) optical sections were obtained through the vascular bed at varying z-intervals in order to perform a computer reconstruction of the complete three-dimensional (3-D) morphology. Image processing algorithms such as gray level thresholding, median filtering, skeletonization, region labeling and template matching were applied to compute the vessel density, lengths and diameters of the neovasculature, and the tortuosity index.
Starting Page	416
Ending Page	420
File Size	504813
Page Count	5
File Format	PDF
ISBN	0818669527
DOI	10.1109/ICIP.1994.413347
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1994-11-13
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Optical filters Optical microscopy Laser transitions Digital images Image analysis Pancreas Rats Optical imaging Fluorescence Probes
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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