NDLI: Analysis of interstitial concentrations of ATP from rat soleus muscle using microdialysis combined with ion-pairing high performance liquid chromatography (HPLC)

Content Provider	IEEE Xplore Digital Library
Author	Jie Tu Fan Yang Guanglin Li Liping Wang
Copyright Year	2009
Description	Author affiliation: Neuroscience Research Center, Shenzhen Institute of Advanced Technology, China Academy of Science, Shenzhen 51806 China (Jie Tu; Guanglin Li; Liping Wang) \|\| Nanomedical Technology Center, Shenzhen Institute of Advanced Technology, China Academy of Sciences, Shenzhen 518067 China (Fan Yang)
Abstract	In the present study, a method is described for the determination of ATP in small samples of interstitial fluid (< 100 μl) using microdialysis combined with ion-pairing high performance liquid chromatography (HPLC) in a gradient elution system which gives a clear separation of ATP from other interstitial constituents. Lactic acid infusion was performed to stimulate ATP to exit the cells to appear in the interstitial space. Microdialysis probe is used to in-vivo sample the molecules in the interstitial fluid. Interstitial ATP could be reliably detected at concentrations higher than $1×10^{-9}$ M. The present study provides a simple, sensitive and selective assay for endogenous extracellular concentrations of ATP from rat soleus muscle.
Starting Page	3787
Ending Page	3789
File Size	520582
Page Count	3
File Format	PDF
ISBN	9781424432967
ISSN	1557170X
DOI	10.1109/IEMBS.2009.5334425
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-09-03
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Performance analysis Muscles Probes Rats Surgery Sugar Circuits USA Councils Extracellular Abdomen pH depression ATP Microdialysis Reversed-phase high-performance liquid chromatography
Content Type	Text
Resource Type	Article
Subject	Computer Vision and Pattern Recognition Health Informatics Signal Processing Biomedical Engineering

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